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k562 cell nuclear extract  (Geneka Biotechnology Inc)

 
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    Geneka Biotechnology Inc k562 cell nuclear extract
    K562 Cell Nuclear Extract, supplied by Geneka Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k562 cell nuclear extract/product/Geneka Biotechnology Inc
    Average 90 stars, based on 1 article reviews
    k562 cell nuclear extract - by Bioz Stars, 2026-03
    90/100 stars

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    Electromobility shift assays (EMSAs) suggest that rs61746197 A > G is a functional SNP. ( A ) Ten micrograms of unstimulated nuclear extract from K562 cells was incubated with reference (Ref, R) or alternative (Alt, A) probe as indicated. Cold Ref probe titrates signal away from Alt at a lower stoichiometric ratio than the converse (arrow and compare lanes 11 – 13 with 6 – 8 ). ( B ) Unstimulated extract ( left panel, U) exhibits a markedly stronger baseline shift than TPA stimulated extract ( right panel, S). Unstimulated nuclear extract (U) when incubated with labeled alternative probe (A) produces a novel secondary shift not observed with either the reference probe (R) or the TPA stimulated extract (S). Addition of RelA–p65 antibody abrogates this secondary shift (arrow).

    Journal: Cold Spring Harbor Molecular Case Studies

    Article Title: 1q21.1 deletion and a rare functional polymorphism in siblings with thrombocytopenia-absent radius–like phenotypes

    doi: 10.1101/mcs.a004564

    Figure Lengend Snippet: Electromobility shift assays (EMSAs) suggest that rs61746197 A > G is a functional SNP. ( A ) Ten micrograms of unstimulated nuclear extract from K562 cells was incubated with reference (Ref, R) or alternative (Alt, A) probe as indicated. Cold Ref probe titrates signal away from Alt at a lower stoichiometric ratio than the converse (arrow and compare lanes 11 – 13 with 6 – 8 ). ( B ) Unstimulated extract ( left panel, U) exhibits a markedly stronger baseline shift than TPA stimulated extract ( right panel, S). Unstimulated nuclear extract (U) when incubated with labeled alternative probe (A) produces a novel secondary shift not observed with either the reference probe (R) or the TPA stimulated extract (S). Addition of RelA–p65 antibody abrogates this secondary shift (arrow).

    Article Snippet: EMSAs were performed using TPA-stimulated and unstimulated nuclear extracts of K562 cells (Active Motif).

    Techniques: Functional Assay, Incubation, Labeling